LOCUS       ISAba14-aphA6-ISAba14_unit4340 bp   DNA    linear       03-OCT-2020
DEFINITION  Pseudomonas aeruginosa.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Unclassified.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of ISAba14-aphA6-ISAba14 unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
     repeat_region   1..54
                     /gene="IRL_ISAba14"
     CDS             77..394
                     /gene="tnpA"
                     /codon_start=1
                     /product="ISAba14 transposase TnpA"
                     /translation="MEYFMARRPRRNHSNDFKAKVALAAIKAEKTLAELSAEFDVHQN
                     QIIDWKNQLISASSQAFDQSKAPTEPPIDLKKLHAKIGEQALEIDFLEGVLKKLGRFN
                     HKS"
     CDS             505..1221
                     /gene="tnpB"
                     /codon_start=1
                     /product="ISAba14 transposase TnpB"
                     /translation="MRCIDELHMQYPFAGSRMMRDLLNRQGHHIGRRHTRTLMKKMGI
                     QALYCKPNLSQANQAQRKYPYLLKGLAIQRSNQVWSTDITYIPMAKGFVYLCAVIDWH
                     SRKVLAHRVSISMEVDFCISALNEAIEKYGRPEIFNTDQGSQFTSDAFIDVLKSNGIQ
                     ISMDGKGRWVDNVMVERLWRSVKYEEVYLKAYSSVTDAKKQLSAYFEFYNLKRPHSSL
                     DKMTPNEFYYDQLPQQNKVA"
     repeat_region   1229..1282
                     /gene="IRR_ISAba14"
     CDS             1436..2215
                     /gene="aphA6"
                     /codon_start=1
                     /product="Aminoglycoside 3'-phosphotransferase"
                     /translation="MELPNIIQQFIGNSVLEPNKIGQSPSDVYSFNRNNETFFLKRSS
                     TLYTETTYSVSREAKMLSWLSEKLKVPELIMTFQDEQFEFMITKAINAKPISALFLTD
                     QELLAIYKEALNLLNSVAIIDCPFISNIDHRLKESKFFIDNQLLDDIDQDDFDAELWG
                     DHKTYLSLWNELTETRVEERLVFSHGDITDSNIFIDKFNEIYFLDLGRAGLADEFVDI
                     SFVERCLREDASEETAKIFLKHLKNDRPDKRNYFLKLDELN"
     repeat_region   3059..3112
                     /gene="IRL_ISAba14"
     CDS             3135..3452
                     /gene="tnpA"
                     /codon_start=1
                     /product="ISAba14 transposase TnpA"
                     /translation="MEYFMARRPRRNHSNDFKAKVALAAIKAEKTLAELSAEFDVHQN
                     QIIDWKNQLISASSQAFDQSKAPTEPPIDLKKLHAKIGEQALEIDFLEGVLKKLGRFN
                     HKS"
     CDS             3563..4279
                     /gene="tnpB"
                     /codon_start=1
                     /product="ISAba14 transposase TnpB"
                     /translation="MRCMDELHMQYPFAGSRMMRDLLNRQGHHIGRRHTRTLMKKMGI
                     QALYCKPNLSQANQAQRKYPYLLKGLAIQRSNQVWSTDITYIPMAKGFVYLCAVIDWH
                     SRKVLAHRVSISMEVDFCISALNEAIEKYGRPEIFNTDQGSQFTSDAFIDVLKSNGIQ
                     ISMDGKGRWVDNVMVERLWRSVKYEEVYLKAYSSVTDAKKQLSAYFEFYNLKRPHSSL
                     DKMTPNEFYYDQLPQQNKVA"
     repeat_region   4287..4340
                     /gene="IRR_ISAba14"
ORIGIN      
        1 agagatggat ccgtaaattt gaacaactcc tataagtgat attctgctcc tcaaatgatg
       61 ttataaacat caatatatgg agtattttat ggcacgtaga ccaagaagaa atcattcaaa
      121 tgattttaaa gctaaggtag cacttgctgc gattaaagca gaaaaaacac ttgctgaatt
      181 gagtgctgag tttgatgttc atcaaaacca aattattgac tggaaaaatc aattgatctc
      241 agcttcctcg caagctttcg atcaatcaaa agctccaaca gaaccaccca tcgatctaaa
      301 aaaactacat gcaaaaatcg gtgagcaggc attagaaatt gattttttag aaggtgtgtt
      361 gaagaaactg ggccgcttca accacaaaag ttaatcgacg actcacttca gatttcagta
      421 tctaagcaag ctaagctgct gaaagtctcc cgtggttgtt attattatcg cccaaaacct
      481 gttagctcat cagatctgaa gctgatgcga tgtattgatg aattacatat gcaatatcct
      541 tttgcaggca gtcgtatgat gcgtgatttg ttgaatcgtc aaggacatca tataggacga
      601 cgtcatacac gtactttaat gaagaaaatg ggtattcagg cgttatattg caaaccaaat
      661 ttaagccagg ctaatcaagc tcagcgtaaa tatccatatc tgctcaaagg gttggctatt
      721 cagcgcagta atcaagtgtg gtctacggat ataacgtata tccctatggc aaaaggcttt
      781 gtttatttat gtgctgtgat tgattggcat agccgcaagg tacttgcgca tagggtatcg
      841 attagtatgg aggtggattt ttgtatttcg gctttaaatg aagcgattga aaaatatggt
      901 cgacctgaaa tatttaatac agaccaaggc agccagttta ccagtgatgc atttattgat
      961 gtattgaaat caaatggcat tcaaatcagt atggatggta aaggtcgatg ggtagataat
     1021 gtgatggttg aacgattatg gcggagcgtt aaatatgaag aggtgtatct caaagcttat
     1081 agcagtgtca cagatgcgaa aaagcaatta agtgcatatt ttgagtttta taatttgaaa
     1141 cgacctcatt cgagtctaga caaaatgaca ccaaatgagt tttactatga tcagctaccc
     1201 caacaaaaca aggtggctta actagagcgg aatatcactt ataaatacgc ttttagttgt
     1261 tcaaacaagt gggaccacct ctgtttatgc aattgaaaga tgatgacaaa attttgaagt
     1321 attgaccatc tatagggaat aagctatatt tttgctacag tgaaccaaat taagatcatc
     1381 tatttactag gccttgcatt tgcggggttt taatgctgaa taaaaggaaa acttgatgga
     1441 attgcccaat attattcaac aatttattgg aaacagtgtt ttagagccaa ataaaattgg
     1501 tcagtcgcca tcggatgttt attcttttaa tcgaaataat gaaacttttt ttcttaagcg
     1561 atctagcact ttatatacag agaccacata cagtgtctct cgcgaagcga aaatgttgag
     1621 ttggctctct gagaaattaa aggtgcctga actcatcatg acttttcagg atgagcagtt
     1681 tgaattcatg atcactaaag cgatcaatgc aaaaccaatt tcagcgcttt ttttaacaga
     1741 ccaagaattg cttgctatct ataaggaggc actcaatctg ttaaattcag ttgctattat
     1801 tgattgtcca tttatttcaa acattgatca tcggttaaaa gagtcaaaat tttttattga
     1861 taaccaactc cttgacgata tagatcaaga tgattttgac gctgaattat ggggagacca
     1921 taaaacttac ctaagtctat ggaatgagtt aactgagact cgtgttgaag aaagattggt
     1981 tttttctcat ggcgatatca cggatagtaa tatttttata gataaattca atgaaattta
     2041 ttttttagat cttggccgtg ctgggttagc agatgaattt gtagatatat cctttgttga
     2101 acgttgccta agagaggatg catcggagga aactgctaaa atatttttaa agcatttaaa
     2161 aaatgataga cctgacaaaa ggaattattt tttaaaactt gatgaattga attgattcca
     2221 agcattatct aaaaatactt aattgtcttt ttacgtcgct aaattttaaa taaataagtg
     2281 aagagcgtta gtggagctat tgatttaaag ttttatatca ccaataaatc taaattttgg
     2341 tttttaaaat gagcttattt aaaatagcta aaactgttta ttagcttaac ttgtgattta
     2401 agtttttttg aatataaaag tgttaattta ttagctttgc aagtttattt aaatttcatc
     2461 gtgcaacatt tttaaccaat tttattatcg atggtatttt tttgtctgct tttcttttct
     2521 tagtctttta tctatctgtc agccatatag tattttatta aattcttatg ggaaatgacg
     2581 aatgttaaat tatcttaaga gctttaataa tatcaatact tatttgattt tatcgataat
     2641 tctgctgtta atcataatat ctctagatta tttctaaact gaatgaatgt ttataatgag
     2701 tgattcatat tgctattgaa atcgccttct cactttgaaa gaaggcgagg atgaggggct
     2761 tttatgttga tttatcgttt taaaaatatc ttataaaaga agcctaatgt gttttcttat
     2821 ataggtttaa acataattgt tgtatatatt aaatctaatt gatcttataa ttttccttta
     2881 tttttttgta ttgttttgca ggtttaaaag tgaaaatttc taaaggatta ttgagtctca
     2941 tcagctattc ttgtttaatc atcagttttc cagtgattgc taaaaccaac acaagtttag
     3001 tttcacctaa aaaaggtgtg atttgtgatc aatatatttg tgtaaataac aaaggtgtag
     3061 agatggatcc gtaaatttga acaactccta taagtgatat tctgctcctc aaatgatgtt
     3121 ataaacatca atatatggag tattttatgg cacgtagacc aagaagaaat cattcaaatg
     3181 attttaaagc taaggtagca cttgctgcga ttaaagcaga aaaaacactt gctgaattga
     3241 gtgctgagtt tgatgttcat caaaaccaaa ttattgactg gaaaaatcaa ttgatctcag
     3301 cttcctcgca agctttcgat caatcaaaag ctccaacaga accacccatc gatctaaaaa
     3361 aactacatgc aaaaatcggt gagcaggcat tagaaattga ttttttagaa ggtgtgttga
     3421 agaaactggg ccgcttcaac cacaaaagtt aatcgacgac tcacttcaga tttcagtatc
     3481 taagcaagct aagctgctga aagtctcccg tggttgttat tactatcgcc caaaacctgt
     3541 gagtgcatca gatctgaagc tgatgcgctg tatggatgaa ttacatatgc aatatccttt
     3601 tgcaggcagt cgtatgatgc gtgatttgtt gaatcgtcaa ggacatcata taggacgacg
     3661 tcatacacgt actttaatga agaaaatggg tattcaggcg ttatattgca aaccaaattt
     3721 aagccaggct aatcaagctc agcgtaaata tccatatctg ctcaaagggt tggctattca
     3781 gcgcagtaat caagtgtggt ctacggatat aacgtatatc cctatggcaa aaggctttgt
     3841 ttatttatgt gctgtgattg attggcatag ccgcaaggta cttgcgcata gggtatcgat
     3901 tagtatggag gtggattttt gtatttcggc tttaaatgaa gcgattgaaa aatatggtcg
     3961 acctgaaata tttaatacag accaaggcag ccagtttacc agtgatgcat ttattgatgt
     4021 attgaaatca aatggcattc aaatcagtat ggatggtaaa ggtcgatggg tagataatgt
     4081 gatggttgaa cgattatggc ggagcgttaa atatgaagag gtgtatctca aagcttatag
     4141 cagtgtcaca gatgcgaaaa agcaattaag tgcatatttt gagttttata atttgaaacg
     4201 acctcattcg agtctagaca aaatgacacc aaatgagttt tactatgatc agctacccca
     4261 acaaaacaag gtggcttaac tagagcggaa tatcacttat aaatacgctt ttagttgttc
     4321 aaacaagtgg gaccacctct
//
